In situ hybridization using radioisotope-labeled probe for GFA-protein messenger RNA - Comparing with immunohistochemistry and northern blot hybridization.
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چکیده
منابع مشابه
In-situ Hybridization to Messenger RNA in Heterodera glycines.
A method is presented for in-situ hybridization to mRNA in second-stage juveniles (J2) of the soybean cyst nematode Heterodera glycines. The protocol was developed using a digoxigenin-labeled RNA probe transcribed from cDNA of a cellulase gene that was known to be expressed in the subventral esophageal glands of H. glycines. Formaldehyde-fixed J2 were cut into sections with a vibrating razor bl...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1988
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.21.175